Research Article
Chitinase Genes LbCHI31 and LbCHI32 from Limonium bicolor Were Successfully Expressed in Escherichia coli and Exhibit Recombinant Chitinase Activities
Table 1
The primers used in cloning the LbCHI31 and LbCHI32 genes into prokaryotic intracellular and extracellular expression vectors.
| Primers names | Primers sequences (5′-3′) | Underlined enzymatic sites |
| inCHI31-R | ATCGGGTACCAGGAGCAGTGCGGTTCTCAAGCCGGT | KpnI | inCHI31-L | CGATGAGCTCAGCAAAAGGCCTCTGGCTATTGC | SacI | inCHI32-R | ATCGCCCGGGCTGGACCTGACGGACCAGCTCGTT | SmaI | inCHI32-L | CGATGGATCCTGTGACGATGCAGAGCCGGATGGGTT | BamHI | exCHI31-R | ATCGGAAGACTGCATGAAAACGACACTCATCCTAACCG | BbsI | exCHI31-L | CGATGGTACCTCAAGCAAAAGGCCTCTGGCTATTG | KpnI | exCHI32-R | ATCGCC ATG GGGAGGCATTGGAGACTGGTAATC | NcoI | exCHI32-L | CGATGGTACCTCAATTATGACGATGCAGAGCCGGAT | KpnI |
|
|