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The Scientific World Journal
Volume 2013 (2013), Article ID 648406, 4 pages
http://dx.doi.org/10.1155/2013/648406
Research Article

Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil

Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, 05508-270 São Paulo, SP, Brazil

Received 31 March 2013; Accepted 30 April 2013

Academic Editors: A. Banerjee, A. Manzin, and A. Zakhartchouk

Copyright © 2013 Paloma de Oliveira Tonietti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated the occurrence of rotavirus in porcine and Rattus norvegicus, at the same time, on a pig farm in the city of Jaguariúna, São Paulo, Brazil. Swine ( ) and rat ( ) fecal samples were analyzed by nested RT-PCR assay. Rotavirus occurred in seven porcine and two rat samples. A total of three pig and one rat samples were further submitted to genetic sequencing. The partial NSP5 gene phylogeny showed that all strains were segregated in the genotype H1. These results point toward a cross-species transmission between rats and pigs on the surveyed farm and represent the first detection of rotavirus in Rattus norvegicus in Brazil.