Genetic Variants of Neurotransmitter-Related Genes and miRNAs in Egyptian Autistic Patients

Table 1

Primer sequences of PCR.

Gene	Sequence	Product size (pb)	Annealing temperature (°C)
MAOA
Forward	5′-CCCAGGCTGCTCCAGAAAC-3′	209, 239, or 269	52
Reverse	5′-GGACCTGGGCAGTTGTGC-3′
MAOB
Forward	5′-GGAACCTCTTATACCACAGG-3′	232	58
Reverse	5′-GACTGCCAGATTTCATCCTC-3′
DRD2
Forward	5′-CCTTCCTGAGTGTCATCAAC-3′	237	54
Reverse	5′-ACGGCTCCTTGCCCTCTAG-3′
miR-431
Forward	5′-GCCTGTAGATCAGGGTCAGG-3′	401	58
Reverse	5′-GACGCTGTGTGAGTTCTTCG-3′
miR-21
Forward	5′-GCCAGAAATGCCTGGGTTT-3′	305	54
Reverse	5′-CAAAAGACTCTAAGTGCCACCA-3′