Genome-Wide Analysis of DNA Methylation in Human Amnion
Figure 4
Methylation-specific PCR (MSP) analysis of SLC30A3. (a) Schematic representation of MSP primer binding sites. Black horizontal arrows: methylated-specific primer (MSPM) binding sites; gray horizontal arrows: unmethylated-specific primer (MSPU) binding sites. The expected PCR product sizes and positions of the primer annealing sites (chromosome and base count, NCBI Build GRCh37/hg19) are indicated. Solid box: coding region; open box: untranslated region. (b) Agarose gel electrophoresis of MSP products. M: product amplified with MSPM; U: product amplified with MSPU. PC: positive control, human methylated DNA standard; NC: negative control, human unmethylated DNA standard; W: water control (details can be found in Section 2). Note that the TL samples show only unmethylated PCR products, while many of the PTL samples show both methylated and unmethylated PCR products, indicative of partial methylation.
