Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages

Figure 2

Effect of sargachromenol on NO production in LPS-stimulated RAW 264.7 cells. Cells were stimulated with 1 μg/mL of LPS only or with LPS plus the indicated concentrations of SM for 24 h. NO (a) levels were determined by the Griess reagent method. PGE₂ (b) levels were measured using an ELISA kit. The data represent the mean ± SD of triplicate experiments. *𝑃<0.05, **𝑃<0.01 versus LPS alone. Cell viability was determined from the 24 h culture of cells stimulated with LPS (1 μg/mL) in the presence of SM. NO: nitric oxide; LPS: lipopolysaccharide; SM: sargachromenol; PGE₂: prostaglandin E₂; ELISA: enzyme-linked immunosorbent assay.

(a)

(b)