Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages
Figure 3
The effect of sargachromenol on the activation of iNOS (a) and COX-2 (b) in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells (5.0 × 105 cells/mL) were stimulated with LPS (1 μg/mL) in the presence of SM at the indicated concentrations for 24 h. Whole-cell lysates (25 μg) were prepared and subjected to 10% SDS-PAGE prior to the analysis of iNOS and β-actin (loading control) expression by Western blotting. iNOS: inducible nitric oxide synthase; COX-2: cyclooxygenase-2; LPS: lipopolysaccharide; SM: sargachromenol; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
