Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages
Figure 4
The effects of sargachromenol on the degradation of IκBα in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells (1.0 × 106 cells/mL) were stimulated with LPS (1 μg/mL) in the presence of the indicated concentrations of SM or PDTC (40 μM) for 15 min. Whole-cell lysates (30 μg) were prepared and subjected to 12% SDS-PAGE prior to analysis of IκBα and β-actin (loading control) expression by western blotting. LPS: lipopolysaccharide; SM: sargachromenol; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; PDTC: pyrrolidine dithiocarbamate.
