Characterization of Human Colorectal Cancer MDR1/P-gp Fab Antibody
Figure 2
Verification of the Fab clones before and after panning by double-enzyme digestion. (a) Before panning, Fab clones (number 1~number 10) were digested by Xba I/Sac I (lanes 1~10) and by Xho I/Spe I (lanes 1′~10′); (b) before panning, Fab clones (number 11~number 20) were digested by Xba I/Sac I (lanes 1~10) and by Xho I/Spe I (lanes 1′~10′); (c) after panning, Fab clones (number 1~number 10) were digested by Xba I/Sac I (lanes 1~10) and Xho I/Spe I (lanes 1′~10′).
