Characterization of Human Colorectal Cancer MDR1/P-gp Fab Antibody

Table 2

The panning results of Fab phage antibody library.

Panning round	Phagemid input (CFU^a/mL)	Phagemid output (CFU^b/mL)	% bound (10^−2)^c	Enrichment^d
1	1.3×10^13	1.46×10^9	1.10
2	1.03×10^12	3.90×10^9	37.90	34.36
3	1.41×10^11	6.38×10^7	4.52	0.12
4	1.06×10^11	4.40×10^7	4.15	0.92
5	6.58×10^10	7.70×10^6	1.17	0.86

aNumber of CFU (colony-forming units) of phagemids incubated with 16-peptide-BSA.
bTotal number of CFUs of phage contained in elutes.
c(Phage output/phage input)/100.
dFold increase in % bound compared to the previous round of panning.