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The Scientific World Journal
Volume 2013, Article ID 716545, 12 pages
http://dx.doi.org/10.1155/2013/716545
Research Article

Characterization of Thermo- and Detergent Stable Antigenic Glycosylated Cysteine Protease of Euphorbia nivulia Buch.-Ham. and Evaluation of Its Ecofriendly Applications

1Department of Biochemistry, National Institute for Research in Reproductive Health (ICMR), Jehangir Merwanji Street, Parel, Mumbai, Maharashtra 400 012, India
2Faculty of Science, Department of Biotechnology, Moolji Jaitha College, North Maharashtra University, Jalgaon, Maharashtra 425002, India

Received 15 August 2013; Accepted 9 September 2013

Academic Editors: A. A. Guevara-Garcia and C. R. C. Calado

Copyright © 2013 Shamkant B. Badgujar and Raghunath T. Mahajan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An antigenic glycosylated cysteine protease has been purified from the latex of Euphorbia nivulia Buch.-Ham. It exhibits remarkable protease activity in the presence of metal ions, oxidizing agents, organic solvents, and detergents. This enzyme showed potential role in leather processing industry due to its dehairing activity for animal hide without hydrolyzing fibrous proteins, producing, by this way, a better quality product. The enzyme can also be used for silver recovering from X-ray plates. In addition, the stability (temperature and surfactants) and hydrolysis of blood stain data also revealed its application in detergent industries. Agriculturally, this protease finds application in biocontrol process against the infectious management of root knot nematode, Meloidogyne incognita. Biologically, it shows noticeable wound healing, haemostatic and antibacterial activity.