Characterization of Thermo- and Detergent Stable Antigenic Glycosylated Cysteine Protease of Euphorbia nivulia Buch.-Ham. and Evaluation of Its Ecofriendly Applications
Table 1
Effect of surfactants, oxidizing agent, and metal ions on proteolytic activity.
Metal ions/O.A./Sur.
Concentration
Residual activity (%)
Control
Nil
100
SDS
1%
51.51 ± 0.18
H2O2
1%
68.18 ± 0.35
Tween 80
1%
100 ± 0.69
Triton X-100
1%
100 ± 1.04
KCl
5 mM
59.64 ± 0.09
NaCl
5 mM
64.97 ± 0.20
ZnCl2
5 mM
58.46 ± 0.16
AgSO4
5 mM
56.69 ± 0.04
CaCl2
5 mM
93.20 ± 0.16
CdCl2
5 mM
53.50 ± 0.29
HgCl2
5 mM
09.43 ± 0.57
FeCl3
5 mM
73.50 ± 0.17
MgCl2
5 mM
97.01 ± 0.22
MnCl2
5 mM
99.89 ± 0.45
Data represented in average values ± SD of experiment, O.A.: oxidizing agent; Sur: surfactants.