High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures

Table 1

Effects of temperature and developmental stage of SEs on induction of secondary SEs in H. dulcis after 6-week culture on MS agar medium without plant growth regulators^X.

Temperature (°C)	Secondary SE induction frequency (%)
	Heart-shaped SEs	Cotyledonary SEs	Germinated SEs
20	0^g	3.3^f	13.3^e
25	0^g	30.0^d	46.7^c
30	10.0^e	66.7^b	86.7^a

XValues with different letters are significantly different according to Duncan’s multiple range test at the 5% level.