High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures

Table 3

Effect of temperature on induction of embryogenic cells from germinated SEs of H. dulcis after 6-week culture on MS liquid medium without plant growth regulators^X.

Temperature (°C)	Embryogenic cell induction frequency (%)^Y	Fresh weight of embryogenic cell clumps (mg/flask)
20	0^c	—
25	65^b	97.3^b
30	100^a	894.6^a

XValues with different letters in a column are significantly different according to Duncan’s multiple range test at the 5% level.
YEmbryogenic cell induction frequency was calculated by Number of flasks formed secondary.
SEs/number of flasks cultured × 100%.