Preamplification Procedure for the Analysis of Ancient DNA Samples

Table 3

qPCR analysis of nonpreamplified and preamplified samples. Ct averages, standard deviations (SD), and coefficients of variation (CV) of mtDNA and AMG-X targets from nonpreamplified and preamplified aDNA samples.

Sample	mtDNA Ct (average ± SD) CV		AMG-X Ct (average ± SD) CV
	Nonpreamplified	Preamplified	Nonpreamplified	Preamplified
B1	37.00 ± 0.28; 0.76%	22.6 ± 0.28; 1.25%	—	—
B2	37.35 ± 0.64; 1.70%	15.71 ± 0.01; —	—	23.62 ± 0.25; 1.05%
B4	34.45 ± 1.06; 3.08%	22.75± 0.07; 0.31%	—	—
B5	36.25 ± 0.42; 1.16%	23.40 ± 0.71; 3.03%	—	25.92 ± 0.05; 0.19%
B7	34.05 ± 0.28; 0.82%	19.85 ± 0.07; 0.35%	—	27.56 ± 0.30; 1.11%
B9	37.5 ± 0.42; 1.13%	25.35 ± 0.07; 0.28%	—	—
A-1V	26.91 ± 0.31; 1.15%	13.19 ± 0.18; 1.36%	35.77 ± 0.09; 0.27%	24.4 ± 0.25; 1.06%
A-2	35.70 ± 0.14; 0.39%	23.85 ± 0.07; 0.29%	—	—
A-3	36.40 ± 0.28; 0.77%	26.40 ± 0.01; —	—	—