Authentication of Nigella sativa Seed Oil in Binary and Ternary Mixtures with Corn Oil and Soybean Oil Using FTIR Spectroscopy Coupled with Partial Least Square
Table 5
PLS performances at some frequency regions for the determination of NSO in ternary mixtures with CO and SO.
Frequencies region (cm−1)
Spectra
Calibration
Validation
RMSEC (% v/v)
RMSEP (% v/v)
4000–650
Normal
0.9975
1.40
0.9986
1.04
Derivative 1
0.9989
0.99
0.9987
0.76
Derivative 2
0.9986
0.97
0.9990
0.69
2985–3024; 752–1755
Normal
0.9985
1.07
0.9989
0.873
Derivative 1
0.9993
0.73
0.9993
0.70
Derivative 2*
0.9945
1.77
0.9852
3.02
2977–3028; 1666–1739; 740–1446
Normal
0.9987
1.07
0.9984
0.97
Derivative 1
0.9993
0.80
0.9986
0.78
Derivative 2
0.9993
0.86
0.9995
0.58
1666–1739
Normal
0.9969
1.56
0.9973
1.27
Derivative 1
0.9978
1.37
0.9964
1.33
Derivative 2
0.9971
1.53
0.9970
1.13
740–1446
Normal
0.9704
4.39
0.9777
3.57
Derivative 1
0.9990
0.88
0.9986
0.89
Derivative 2
0.9954
1.55
0.9991
0.90
*The frequency region and FTIR spectral treatment selected for quantification were marked with italics.
