Research Article
Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis
Table 2
Immunoreagents, working dilutions, and sources of antibodies used in the present study.
| | | Working dilution | Antibody suppliers (reference) |
| | Primary antibody | | | | Rabbit anti-bovine rod opsin polyclonal antibody, CERN-922 | 1 : 1000 | Gift from Dr. Willem J. DeGrip | | Mouse anti-Islet-1 monoclonal antibody (clone 39.4D5) | 1 : 200 | Developmental Studies Hybridoma Bank (DSHB) | | Mouse anti-SV2 monoclonal antibody | 1 : 200 | DSHB | | Rabbit anti-calbindin polyclonal antibody | 1 : 1000 | Swant (Ref. CB-38a) | | Rabbit anti-calretinin polyclonal antibody | 1 : 2000 | Swant (Ref. 7699-4) | | Secondary antibodies | | | | Alexa Fluor 594 goat-anti-mouse IgG antibody | 1 : 200 | Molecular Probes, The Netherlands | | Alexa Fluor 488 goat-anti-rabbit IgG antibody | 1 : 200 | Molecular Probes, The Netherlands | | Anti-mouse IgG biotin conjugate | 1 : 200 | Sigma (B 7264) |
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