Oncogene- and Oxidative Stress-Induced Cellular Senescence Shows Distinct Expression Patterns of Proinflammatory Cytokines in Vascular Endothelial Cells

Figure 3

Real time PCR analysis of the expression of proinflammatory cytokines in HUVECs. HUVECs were infected with AdGFP or AdRas12V or treated with 100 μmol/L H₂O₂. After 1 week, total RNA was extracted for real time PCR analysis. The expression of IL1-β, IL-6, and NGF was normalized to GAPDH. * and **: 𝑃<0.05 and 𝑃<0.01, respectively, versus AdGFP infection. †: 𝑃<0.01 versus AdRas12V infection (𝑛=6 per group).

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