The Scientific World Journal / 2013 / Article / Fig 4

Research Article

Oncogene- and Oxidative Stress-Induced Cellular Senescence Shows Distinct Expression Patterns of Proinflammatory Cytokines in Vascular Endothelial Cells

Figure 4

The expression of IL-1β and NGF proteins in HUVECs treated with AdRas12V or H2O2. (a) Time course of the accumulation of IL-1β and NGF in the culture media. HUVECs were infected with AdGFP (closed squares) or AdRas12V (closed circles) or treated with H2O2 (open circles). After 1 week, HUVECs were washed with PBS, and medium was replaced with serum free Dulbecco’s modified Eagle medium (DMEM). HUVECs were incubated for the indicated periods, and culture media were collected for ELISA. * and **: and , respectively, versus AdGFP infection at each time point. †: versus H2O2 treatment at each time point ( per group). (b) The effect of BAY11-7082 on cytokine production. Experiments were performed as in (a). In addition, some HUVECs were preincubated with 10 μmol/L of BAY11-7082 (Bay) for 2 hrs and then washed with PBS. The medium was replaced with serum free DMEM. HUVECs were then incubated for 6 hrs in the presence or absence of Bay ( per group).

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