Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR

Table 2

Number of shoots/plant obtained from different tissue culture techniques on different garlic species.

Explant Type	First micropropagation (shoots/plant)			Sub-culture (shoots/plant)
	Medium 1	Medium 2	LSD (5%)	Medium 1	Medium 2	LSD (5%)
Meristem culture (AT)	4.63^b	14.10^a	0.86	3.93^b	13.27^a	1.86
Shoot tip culture (AT)	2.41^b	11.37^a	1.73	2.36^b	8.93^a	2.16
Meristem culture (AS)	5.41^b	11.67^a	1.21	5.56^b	11.61^a	2.58
Shoot tip culture (AS)	2.98^b	9.84^a	1.73	3.61^b	10.17^a	2.16

AT: A. tuncelianum; AS: A. sativum.
^a,bMeans followed by different letters in the same column are significantly different regarding Tukey’s multiple range test at 𝑃<0.05.