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The Scientific World Journal
Volume 2013, Article ID 781301, 11 pages
http://dx.doi.org/10.1155/2013/781301
Research Article

Actinomycetes Diversity among rRNA Gene Clones and Cellular Isolates from Sambhar Salt Lake, India

1National Bureau of Agriculturally Important Microorganisms, Kushmaur, Katholi, Maunath Bhanjan, Uttar Pradesh 275101, India
2National Bureau of Agriculturally Important Insects, H.A. Farm Post, Bellary Road, Bangalore, Karnatka 560024, India
3Sher-e-Kasmir University of Agriculture Science & Technology of Jammu, Main Campus, Chatta, Jammu (J&K) 180009, India

Received 3 August 2013; Accepted 11 September 2013

Academic Editors: H. Takami and J. Yoon

Copyright © 2013 A. K. Yadav et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The vertical stratification of actinomycetes diversity in Sambhar salt lake (India’s largest salt lake) was investigated by using cultivable and uncultivable approaches. The isolates from cultured approaches were clustered on the basis of cultural, morphological, biochemical, and cell wall characteristics, and results were further strengthened by 16S rDNA-RFLP into five major groups. 16S rDNA sequencing of the representative isolates from each clusters was identified as belonging to Streptomyces, Actinopolyspora, Microbispora, Saccharopolyspora, and Actinoplanes genera, while culture independent group was established as Streptomyces (130 clones, 20 OTUs), Micromonospora (96 clones, 7 OTUs), Streptosporangium (79 clones, 9 OTUs), Thermomonospora (46 clones, 8 OTUs), and Dactylosporangium (58 clones, 8 OTUs). The diversity assessment using Shannon and Wiener index was found to be 1.55, 1.52, 1.55, and 1.49 from surface lake water, at depth of 1.5 m, shallow layer of water with algal population, and finally at depth of 2.5 m, respectively. We observed diversity in terms of the species richness as Streptomyces is dominant genus in both culture dependent and culture independent techniques followed by Microbispora (culture dependent methods) and Micromonospora (culture independent method) genera, respectively.