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The Scientific World Journal
Volume 2013, Article ID 878735, 9 pages
http://dx.doi.org/10.1155/2013/878735
Research Article

Evaluation of the Antibacterial and Antifungal Potential of Peltophorum africanum: Toxicological Effect on Human Chang Liver Cell Line

1Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of Biochemistry & Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 570, South Africa
2Department of Microbiology and Parasitology, Faculty of Science, University of Buea, P.O. Box 63, Buea, Cameroon

Received 3 January 2013; Accepted 28 January 2013

Academic Editors: J.-T. Cheng and P. Cos

Copyright © 2013 Benjamin I. Okeleye et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We assessed the in vitro antimicrobial activity of Peltophorum africanum by means of the agar well and macrodilution methods. The toxicity on a normal human liver cell (Chang liver cell) was determined using the CellTiter-Blue cell viability assay, and the compounds contained in the fractions were identified using GC-MS. Zone diameter of inhibition of the extract ranged from  mm for bacteria and from    mm for yeast. Marked activity of the extract was observed against Plesiomonas shigelloides ATCC 51903, with MIC and MLC values of 0.15625 and 0.3125 mg/mL, respectively. The extract was both bactericidal ( ) and bacteriostatic/fungistatic ( ) in activity. Lethal dose at 50 (LD50) showed degree of toxicity at 24 hrs, and 95 percentile of cell death dose activity ranged from log . The activity of the eight fractions tested ranged from  mg/mL (IC50) and from    mg/mL (IC90). The extract was toxic to human Chang liver cell lines.