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The Scientific World Journal
Volume 2013 (2013), Article ID 925375, 6 pages
Research Article

Comparison of Biofilm and Attachment Mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniae

1Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Avenida Marechal Campos 1468, 29040-090 Vitória, ES, Brazil
2Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo, Campus Vitória, Avenida Vitória 1729, 29040-780 Vitória, ES, Brazil
3Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo, Campus Vila Velha, Avenida Ministro Salgado Filho S/Nº, 29106-010 Vila Velha, ES, Brazil
4Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural do Espírito Santo, INCAPER, Rua Afonso Sarlo 160, 29052-010 Vitória, ES, Brazil

Received 22 August 2013; Accepted 16 September 2013

Academic Editors: H. P. Bais and V. Fedorenko

Copyright © 2013 Adriana Marcia Nicolau Korres et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Some bacterial species can colonize humans and plants. It is almost impossible to prevent the contact of clinically pathogenic bacteria with food crops, and if they can persist there, they can reenter the human food chain and cause disease. On the leaf surface, microorganisms are exposed to a number of stress factors. It is unclear how they survive in such different environments. By increasing adhesion to diverse substrates, minimizing environmental differences, and providing protection against defence mechanisms, biofilms could provide part of the answer. Klebsiella pneumoniae subsp. pneumoniae is clinically important and also associated with fruit diseases, such as “pineapple fruit collapse.” We aimed to characterize biofilm formation and adhesion mechanisms of this species isolated from pineapple in comparison with a clinical isolate. No differences were found between the two isolates quantitatively or qualitatively. Both tested positive for capsule formation and were hydrophobic, but neither produced adherence fibres, which might account for their relatively weak adhesion compared to the positive control Staphylococcus epidermidis ATCC 35984. Both produced biofilms on glass and polystyrene, more consistently at 40°C than 35°C, confirmed by atomic force and high-vacuum scanning electron microscopy. Biofilm formation was maintained in an acidic environment, which may be relevant phytopathologically.