Research Article
Effect of Polyunsaturated Fatty Acids on Homocysteine Metabolism through Regulating the Gene Expressions Involved in Methionine Metabolism
Table 1
The primers used in real time PCR.
| | GenBank Acc. | Symbol | Primer |
| | NM_030850.1 | Bhmt | F: TGCGCAGTGCGTTTGGTAA | R: AGGGCGTGGCTCATCAAGTAAG | | NM_019179.1 | Tyms | F: AAGAATCATCATGTGTGCCTGGAA | R: TCCTGACCGCTGGTAAAGCTG | | NM_017201.1 | Sahh | F: TGTGGACCCACCCAGACAAATA | R: CAGCTTGGTCAGCTTGACGTTC | | NM_017127.1 | Chka | F: GGACCAGTTCCACATCAGTGTCA | R: GAGGTTCATCACCAACACTGGCTA | | NM_017084.1 | Gnmt | F: GCCAGCGACAAGATGCTGAA | R: GACAGCGTCAAAGCCATCTCC | | NM_017074.1 | Cse | F: CAGTGATGTTGTCATGGGCTTAGTG | R: CATCCGGATCTGCAGTGTCTTC | | NM_013003.1 | Pemt | F: AGGAGTCCAGAGTGACCACATTTC | R: CACGTAGACGAGTGCCACCA | | NM_012860.2 | Mat1a | F: CCGAGCGAGAGCTACTAGAGGTTG | R: CCGAAATGACCATAGCATGCAG | | NM_012522.2 | Cbs | F: TGCATTATCGTGATGCCTGAGAA | R: GGGAATCGAATCTGGCGTTG | | NM_001107894.1 | Etnk1 | F: TGAGTTTATCCAGGGTGAAGCTTTG | R: TCCAGCCATTGTGTGCATGA | | NM_001039003.1 | Mtrr | F: CAAAGTATGTGCAAGACAACCTCCA | R: TGATTTCTACAAGGGCGTCGTG | | NM_001009349.1 | Mthfs | F: AATCACATGGACATGGTGAGGCTA | R: CAGCCGGTTGCCATCTTTG | | NM_001007699.1 | Cept1 | F: TGCAAGGATACTGGGAATGGCTA | R: AGGCGATGTAAGCCCACAGAG | | NM_198731.2 | Chdh | F: CATCCCTGTGGTGTGCCATC | R: TGTGTGCAAGCATGCTGAATGTA | | NM_030864.1 | Mtr | F: ACTTGCGCAAACTCCGCTATG | R: TGCCAAGGATTCTGTCAACCTG | | NM_022698.1 | Bad | F: GGCAGCCAATAACAGTCATCA | R: GGTACGAACTGTGGCGACTC | | XM_342975.4 | Mthfr | F: AGCTTGAAGCCACCTGGACTGTAT | R: AGACTAGCGTTGCTGGGTTTCAGA | | NM_031144.2 | Actb | F: GGAGATTACTGCCCTGGCTCCTA | R: GACTCATCGTACTCCTGCTTGCTG |
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Mthfr: methylenetetrahydrofolate reductase; Sahh: S-adenosylhomocysteine hydrolase; Bhmt: betaine-homocysteine methyltransferase; Cbs: cystathionine beta synthase; Mat1a: methionine adenosyltransferase I, alpha; Pemt: phosphatidylethanolamine N-methyltransferase; Cse: cystathionine gamma-lyase; Chka: choline kinase alpha; Tyms: thymidylate synthetase; Chdh: choline dehydrogenase; Gnmt: glycine N-methyltransferase; Mthfs: 5,10-methenyltetrahydrofolate synthetase; Mtr: 5-methyltetrahydrofolate-homocysteine methyltransferase; Cept1: choline/ethanolamine phosphotransferase 1; Mtrr: 5-methyltetrahydrofolate-homocysteine methyltransferase reductase; Bad: betaine aldehyde dehydrogenase; Etnk1: ethanolamine kinase 1.
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