A TaqMan Real-Time PCR Assay for Detection and Quantification of Sporisorium scitamineum in Sugarcane

<table>Sensitivity test of conventional PCR based on eight ten-fold serial dilutions of smut pbE DNA. M: 100 bp DNA ladder marker; Lane 1, 100 pg/μL; Lane 2, 10 pg/μL; Lane 3, 1 pg/μL; Lane 4, 100 fg/μL; Lane 5, 10 fg/μL; Lane 6, 1 fg/μL; Lane 7, 100 ag/μL; Lane 8, 10 ag/μL.</table>

The Scientific World Journal

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Figure 2

Figure 2: A TaqMan Real-Time PCR Assay for Detection and Quantification of Sporisorium scitamineum in Sugarcane