A TaqMan Real-Time PCR Assay for Detection and Quantification of <i>Sporisorium scitamineum</i> in Sugarcane

<table>Sensitivity test of conventional PCR based on five-fold serial dilutions of +1 leaf gDNA. M: 100 bp DNA ladder marker; Lane 1, 500 ng/<i>μ</i>L; Lane 2, 100 ng/<i>μ</i>L; Lane 3, 20 ng/<i>μ</i>L; Lane 4, 4 ng/<i>μ</i>L; Lane 5, 0.8 ng/<i>μ</i>L; Lane 6, positive control; Lane 7, mock control; Lane 8, blank control.</table>

The Scientific World Journal

fig3

Figure 3

Figure 3: A TaqMan Real-Time PCR Assay for Detection and Quantification of <i>Sporisorium scitamineum</i> in Sugarcane 

The Scientific World Journal

A TaqMan Real-Time PCR Assay for Detection and Quantification of Sporisorium scitamineum in Sugarcane

Figure 3