A TaqMan Real-Time PCR Assay for Detection and Quantification of Sporisorium scitamineum in Sugarcane

Table 1

Mean Ct values and copy numbers obtained by sensitivity test by TaqMan real-time PCR assay.

Concentration of +1 leaf DNA (ng/μL)	Ct values (Ma±𝜎)	Copy numbers (Ma±𝜎) (copies/μL)
500	28.202±0.354	12247.062±1579.742
100	30.700±0.391	2246.733±349.973
20	32.895±0.456	509.013±101.842
4	35.561±0.200	81.431±2.540
0.8	36.575±0.330	41.123±4.953

Ma = mean value of three technical replicates; 𝜎 = standard error.