Comparative Analyses of Response Surface Methodology and Artificial Neural Network on Medium Optimization for Tetraselmis sp. FTC209 Grown under Mixotrophic Condition

Table 6

Validation results of medium compositions as suggested by RSM and ANN models.

Tetraselmis sp. FTC 209 cultivation performance	Optimum points prediction		W-30 mixotrophic	Walne’s autotrophic
	RSM	ANN
Glucose (g/L)	28.11	24.05	30	—
Sodium Nitrate (g/L)	5.10	4.70	5.24	0.10
Yeast Extract (g/L)	1.20	0.93	—	—
Predicted Prlipid (mg/L per day)	169.20	175.84	—	—
Observed Prlipid (mg/L per day)	160.17	173.11	90.90	4.82
𝑋max (g/L)	11.86	12.38	8.10	0.830
𝑃max/𝑋max (mg/g dcw)	189.07	195.77	224.78	122.30