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The Scientific World Journal
Volume 2014, Article ID 102541, 27 pages
http://dx.doi.org/10.1155/2014/102541
Research Article

Expression Profiling Using a cDNA Array and Immunohistochemistry for the Extracellular Matrix Genes FN-1, ITGA-3, ITGB-5, MMP-2, and MMP-9 in Colorectal Carcinoma Progression and Dissemination

1Postgraduate Program in Interdisciplinary Surgery Science, UNIFESP-Escola Paulista de Medicina, São Paulo, SP 04023-062, Brazil
2Research and Teaching Unit of Hospital Municipal Dr. Munir Rafful, Volta Redonda, RJ 27277-130, Brazil
3Hospital de Cancer de Barretos-Fundação Pio XII, Barretos, SP 14784-400, Brazil
4Genetics Laboratory-GRAAAC, UNIFESP-Escola Paulista de Medicina, São Paulo, SP 04023-062, Brazil

Received 20 November 2013; Accepted 13 January 2014; Published 4 March 2014

Academic Editors: D. Morris and E. Tagliabue

Copyright © 2014 Suzana Angelica Silva Lustosa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Colorectal cancer dissemination depends on extracellular matrix genes related to remodeling and degradation of the matrix structure. This investigation intended to evaluate the association between FN-1, ITGA-3, ITGB-5, MMP-2, and MMP-9 gene and protein expression levels in tumor tissue with clinical and histopathological neoplastic parameters of cancer dissemination. The expression associations between ECM molecules and selected epithelial markers EGFR, VEGF, Bcl2, P53, and KI-67 have also been examined in 114 patients with colorectal cancer who underwent primary tumor resection. Quantitative real-time PCR and immunohistochemistry tissue microarray methods were performed in samples from the primary tumors. The gene expression results showed that the ITGA-3 and ITGB-5 genes were overexpressed in tumors with lymph node and distant metastasis (III/IV-stage tumors compared with I/II tumors). The MMP-2 gene showed significant overexpression in mucinous type tumors, and MMP-9 was overexpressed in villous adenocarcinoma histologic type tumors. The ECM genes MMP9 and ITGA-3 have shown a significant expression correlation with EGFR epithelial marker. The overexpression of the matrix extracellular genes ITGA-3 and ITGB-5 is associated with advanced stage tumors, and the genes MMP-2 and MMP-9 are overexpressed in mucinous and villous adenocarcinoma type tumors, respectively. The epithelial marker EGFR overactivity has been shown to be associated with the ECM genes MMP-9 and ITGA-3 expression.