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The Scientific World Journal
Volume 2014, Article ID 121760, 4 pages
Research Article

Evaluation of the Inhibition of Carbohydrate Hydrolyzing Enzymes, the Antioxidant Activity, and the Polyphenolic Content of Citrus limetta Peel Extract

1Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Avenida Normalistas 800, Colinas de la Normal, 44270 Guadalajara, JAL, Mexico
2Department of Chemistry, Lagos State University, PMB 0001, Lasu Post Office, Ojo, Lagos, Nigeria
3Lancaster Medical School, Lancaster University, Lancaster LA1 4YG, UK

Received 1 August 2014; Revised 25 November 2014; Accepted 28 November 2014; Published 21 December 2014

Academic Editor: Walter Hunziker

Copyright © 2014 Eduardo Padilla-Camberos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Type 2 diabetes mellitus is one of the most frequent causes of death in Mexico, characterized by chronic hyperglycemia. One alternative strategy for this metabolic abnormality is inhibiting the enzymes responsible for the metabolism of carbohydrates. We evaluated whether the aqueous Citrus limetta peel extract could inhibit the metabolism of carbohydrates. We found that this extract inhibited primarily the enzyme α-amylase by 49.6% at a concentration of 20 mg/mL and to a lesser extent the enzyme α-glucosidase with an inhibition of 28.2% at the same concentration. This inhibition is likely due to the high polyphenol content in the Citrus limetta peel (19.1 mg GAE/g). Antioxidant activity of the Citrus limetta peel demonstrated dose-dependent antioxidant activity, varying from 6.5% at 1.125 mg/mL to 42.5% at 20 mg/mL. The study of these polyphenolic compounds having both antihyperglycemic and antioxidant activities may provide a new approach to the management of type 2 diabetes mellitus.