Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest

Figure 11

(a) CKI upregulation by AAA induces cell cycle arrest in COLO-205 cells. Agarose gel electrophoresis of PCR products of (A) β-actin, (B) p16, (C) p21, and (D) p27 genes. (b) The fold change in expression of CKI’s gene after AAA treatment. The densitometric analysis was done of the PCR products p16, p21, and p27 gene to measure the fold change in each gene and was normalised to house-keeping gene β-actin. Data presented as fold change in gene expression between AAA treated cells and untreated control cells. ; .

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