Adaptation of Mycoplasmas to Antimicrobial Agents: Acholeplasma laidlawii Extracellular Vesicles Mediate the Export of Ciprofloxacin and a Mutant Gene Related to the Antibiotic Target

Table 1

Ciprofloxacin content and bacteriostatic activities of the extracellular vesicles of Acholeplasma laidlawii PG8R.

Test samples	Ciprofloxacin (5 g/mL)	Extracellular vesicles of A. laidlawii PG8R*
		Native	Destroyed
Ciprofloxacin content, ng/mg of protein	—	—	0.66±0.15
Lysis area (mm)**	21.2±0.3	3.4±0.21; (𝑃<0.05)	8.8±0.16; (𝑃<0.05)

∗Mean ± standard deviation.
**Native extracellular vesicles of A. laidlawii PG8R were used as a control to estimate the bacteriostatic effects of the vesicle components.