Effect of Simulated Gastrointestinal Conditions on Biofilm Formation by Salmonella 1,4,[5],12:i:-

Figure 1

Time course of biofilm production by 133 Salmonella Typhimurium 1,4,[5],12:i:- isolates using an Alamar Blue microtiter assay applied in different incubation conditions. Mean and standard deviation for MHB were at 24 h 0,856 ± 0,095, at 48 h 0,977 ± 0,105, and at 72 h 1,044 ± 0,118. SIF under static conditions were at 24 h 0,531 ± 0,217, at 48 h 0,443 ± 0,222, and at 72 h 0,409 ± 0,146. SIF under agitation conditions were at 24 h 0,377 ± 0,136, at 48 h 0,355 ± 0,142, and at 72 h 0,297 ± 0,108. MHB: Mueller Hinton Broth; SIF: simulated intestinal fluid.