Effect of Simulated Gastrointestinal Conditions on Biofilm Formation by Salmonella 1,4,[5],12:i:-

Table 1

Characterization of biofilm-forming ability of 133 Salmonella Typhimurium 1,4,[5],12:i:- isolates using an Alamar Blue microtiter assay applied in different incubation conditions.

Biofilm formation ability	Number of strains that produced biofilm (𝑛, %)
	24 h			48 h			72 h
	MHB	SIF with static conditions	SIF with dynamic conditions	MHB	SIF with static conditions	SIF with dynamic conditions	MHB	SIF with static conditions	SIF with dynamic conditions
Strong biofilm producer	0.0%	21.8% (𝑛=29)	0.8% (𝑛=1)	0.0% (𝑛=0)	9.0% (𝑛=12)	0.8% (𝑛=1)	37.6% (𝑛=50)	6.8% (𝑛=9)	3.0% (𝑛=4)
Moderate biofilm producer	54.1% (𝑛=72)	42.1% (𝑛=46)	15.8% (𝑛=21)	66.2% (𝑛=88)	39.8% (𝑛=53)	37.6% (𝑛=50)	61.7% (𝑛=82)	52.6% (𝑛=70)	39.1% (𝑛=52)
Weak biofilm producer	45.9% (𝑛=61)	36.1% (𝑛=58)	83.5% (𝑛=111)	33.8% (𝑛=45)	51.1% (𝑛=68)	61.7% (𝑛=82)	0.8% (𝑛=1)	40.6% (𝑛=54)	57.9% (𝑛=77)

MHB: Mueller Hinton Broth; SIF: simulated intestinal fluid.