Research Article

CMYB1 Encoding a MYB Transcriptional Activator Is Involved in Abiotic Stress and Circadian Rhythm in Rice

Figure 6

Transcriptional activity analysis of CMYB1 in rice protoplast via dual luciferase reporter assay system. (a) Construction of reporter, effector, and internal control vectors. (b) The luciferase activities. Vector control contained reporter and internal control plasmids; DREB1A contained reporter, internal control, and 35S::DREB1A protein plasmids; DREB1A+GAL4DBD contained reporter, internal control, 35S::DREB1A protein, and 35S::GAL4DBD protein plasmids; DREB1A+G-CMYB1 contained reporter, internal control, 35S::DREB1A protein, and 35S::GAL4DBD-CMYB1 fusion protein plasmids; DREB1A+G-ZFP179 contained reporter, internal control, 35S::DREB1A protein, and 35S::GAL4DBD-ZFP179 fusion protein plasmids.
178038.fig.006a
(a)
178038.fig.006b
(b)