Production, Characterization of Tannase from Penicillium montanense URM 6286 under SSF Using Agroindustrial Wastes, and Application in the Clarification of Grape Juice (Vitis vinifera L.)

Table 3

Experimental matrix Plackett-Burman planning (PB) for the production of tannase from Penicillium montanense URM 6286 under FES using agroindustrial wastes.

Trials	Time	Temperature	Moisture	pH	Tannic acid	Yeast extract	Urea	Glucose	Starch	Manganese sulfate	Monobasic potassium phosphate
1	120 (+)	36 (+)	35 (−)	8 (+)	0.5 (−)	0.25 (−)	0.25 (−)	0.5 (+)	0.5 (+)	0.1 (+)	0.1 (−)
2	120 (+)	36 (+)	35 (−)	8 (+)	2 (+)	0.25 (−)	0.5 (+)	0.1 (−)	0.1 (−)	0.05 (−)	0.5 (+)
3	72 (−)	36 (+)	65 (+)	5 (−)	2 (+)	0.25 (−)	0.25 (−)	0.1 (−)	0.5 (+)	0.1 (+)	0.5 (+)
4	72 (−)	28 (−)	65 (+)	8 (+)	2 (+)	0.25 (−)	0.5 (+)	0.5 (+)	0.1 (−)	0.1 (+)	0.1 (−)
5	120 (+)	36 (+)	65 (+)	5 (−)	2 (+)	0.5 (+)	0.25 (−)	0.5 (+)	0.1 (−)	0.05 (−)	0.1 (−)
6	72 (−)	28 (−)	35 (−)	5 (−)	0.5 (−)	0.25 (−)	0.25 (−)	0.1 (−)	0.1 (−)	0.05 (−)	0.1 (−)
7	120 (+)	28 (−)	65 (+)	5 (−)	0.5 (−)	0.25 (−)	0.5 (+)	0.5 (+)	0.5 (+)	0.05 (−)	0.5 (+)
8	72 (−)	28 (−)	35 (−)	8 (+)	2 (+)	0.5 (+)	0.25 (−)	0.5 (+)	0.5 (+)	0.05 (−)	0.5 (+)
9	120 (+)	28 (−)	65 (+)	8 (+)	0.5 (−)	0.5 (+)	0.25 (−)	0.1 (−)	0.1 (−)	0.1 (+)	0.5 (+)
10	72 (−)	36 (+)	65 (+)	8 (+)	0.5 (−)	0.5 (+)	0.5 (+)	0.1 (−)	0.5 (+)	0.05 (−)	0.1 (−)
11	72 (−)	36 (+)	35 (−)	5 (−)	0.5 (−)	0.5 (+)	0.5 (+)	0.5 (+)	0.1 (−)	0.1 (+)	0.5 (+)
12	120 (+)	28 (−)	35 (−)	5 (−)	2 (+)	0.5 (+)	0.5 (+)	0.1 (−)	0.5 (+)	0.1 (+)	0.1 (−)