Production, Characterization of Tannase from <i>Penicillium montanense</i> URM 6286 under SSF Using Agroindustrial Wastes, and Application in the Clarification of Grape Juice (<i>Vitis vinifera</i> L.)

<table class="table-group" id="tab4"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Variables</td><td align="center">Effect</td><td align="center">Coefficient</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">(1) Time</td><td align="center">−0.67538</td><td align="center">−0.337691</td></tr><tr><td align="left">(2) Temperature</td><td align="center"><span style="width: 50.3116ptpx;"><svg height="10.7666pt" id="M19" style="vertical-align:-0.2402pt" version="1.1" viewbox="-0.0498162 -10.5264 50.3116 10.7666" width="50.3116pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M306 382C339 403 362 417 379 433C398 450 411 473 411 500C411 578 352 635 248 635H247C181 635 134 604 106 573L56 506L82 482C117 529 151 557 201 557C246 557 287 531 287 466C287 404 228 364 143 336L150 302C165 306 186 309 204 309C258 309 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264 327 174C327 100 297 45 235 45C178 45 130 84 106 106C93 117 82 120 70 112C53 99 38 76 38 61S43 36 63 20C84 2 126 -12 166 -12C226 -11 297 15 354 56C424 105 455 164 455 226C455 312 382 394 254 394C228 394 175 380 125 362L152 515Z" id="g13-51"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,23.411,0)"><path d="M440 175C403 110 395 108 335 108H173L293 222C391 316 432 379 432 457C432 568 349 635 250 635C187 635 137 610 100 573L36 479L65 456C97 502 138 544 199 544C268 544 303 495 303 425C303 349 264 274 194 191C145 133 86 78 33 26V0H434C445 42 457 105 473 164L440 175Z" id="g13-48"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,30.185,0)"><path d="M476 623H66C59 580 53 527 39 449H74C102 508 110 515 178 515H391C305 352 205 176 87 1L95 -12L198 0C289 205 390 410 485 611L476 623Z" id="g13-53"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,37.098,0)"><path d="M440 175C403 110 395 108 335 108H173L293 222C391 316 432 379 432 457C432 568 349 635 250 635C187 635 137 610 100 573L36 479L65 456C97 502 138 544 199 544C268 544 303 495 303 425C303 349 264 274 194 191C145 133 86 78 33 26V0H434C445 42 457 105 473 164L440 175Z" id="g13-48"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0095,0,0,-0.0095,43.872,-5.984)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z" id="g50-43"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="611" vert-adv-y="611"></glyph.data></g></svg></span></td><td align="center">1.876362</td></tr><tr><td align="left">(3) Moisture</td><td align="center"><span style="width: 50.3116ptpx;"><svg height="10.7666pt" id="M20" style="vertical-align:-0.2402pt" version="1.1" viewbox="-0.0498162 -10.5264 50.3116 10.7666" width="50.3116pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M409 0V34C329 39 322 56 322 125V635C234 611 160 593 94 583V554L145 551C186 547 190 541 190 488V125C190 54 181 39 102 34V0H409Z" id="g13-47"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,6.774,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z" id="g113-47"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="228" vert-adv-y="228"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,9.863,0)"><path d="M253 635C100 635 24 509 24 420C24 314 97 231 217 231C234 231 251 236 275 248L324 274C298 154 195 47 47 21L53 -15C98 -14 184 2 223 18C365 74 471 201 471 382C471 519 398 635 253 635ZM232 595C310 595 335 485 335 387C335 365 334 340 330 313C316 307 300 300 275 300C202 300 156 374 156 460C156 525 178 595 232 595Z" id="g13-55"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,16.637,0)"><path d="M179 359C209 485 294 542 343 567C378 584 421 597 450 603L443 641C405 636 339 624 294 609C178 570 28 456 28 243C28 84 127 -12 253 -12C385 -12 480 89 480 215C480 328 403 402 288 402C272 402 254 394 229 382L179 359ZM234 331C302 331 349 265 349 171C349 106 335 29 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204 32 157C32 55 118 -12 249 -12C346 -12 472 51 472 178C472 266 409 313 339 351C378 376 396 392 412 406C435 428 445 453 445 480C445 569 371 635 262 635ZM246 595C286 595 338 561 338 475C338 417 318 386 301 370C224 406 172 439 172 507C172 549 199 595 246 595ZM260 28C210 28 152 72 152 169C152 221 175 265 204 287C301 235 350 209 351 137C352 71 315 28 260 28Z" id="g13-54"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0095,0,0,-0.0095,43.872,-5.984)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z" id="g50-43"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="611" vert-adv-y="611"></glyph.data></g></svg></span></td><td align="center">0.980392</td></tr><tr><td align="left">(4) pH</td><td align="center">−1.90632</td><td align="center">−0.953159</td></tr><tr><td align="left">(5) Tannic acid</td><td align="center"><span style="width: 50.3116ptpx;"><svg height="10.7666pt" id="M21" style="vertical-align:-0.2402pt" version="1.1" viewbox="-0.0498162 -10.5264 50.3116 10.7666" width="50.3116pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M306 382C339 403 362 417 379 433C398 450 411 473 411 500C411 578 352 635 248 635H247C181 635 134 604 106 573L56 506L82 482C117 529 151 557 201 557C246 557 287 531 287 466C287 404 228 364 143 336L150 302C165 306 186 309 204 309C258 309 324 275 324 179C324 85 285 44 235 44C175 44 128 88 106 108C92 118 80 116 69 106C56 97 39 79 39 61C39 47 46 34 63 21C83 5 123 -12 174 -12C236 -12 454 58 454 222C454 297 403 362 306 380V382Z" id="g13-49"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,6.774,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z" id="g113-47"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="228" vert-adv-y="228"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,9.863,0)"><path d="M409 0V34C329 39 322 56 322 125V635C234 611 160 593 94 583V554L145 551C186 547 190 541 190 488V125C190 54 181 39 102 34V0H409Z" id="g13-47"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,16.637,0)"><path d="M152 515H407L439 613L433 623H115L70 303C91 310 134 322 179 322C270 322 327 264 327 174C327 100 297 45 235 45C178 45 130 84 106 106C93 117 82 120 70 112C53 99 38 76 38 61S43 36 63 20C84 2 126 -12 166 -12C226 -11 297 15 354 56C424 105 455 164 455 226C455 312 382 394 254 394C228 394 175 380 125 362L152 515Z" id="g13-51"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,23.411,0)"><path d="M306 382C339 403 362 417 379 433C398 450 411 473 411 500C411 578 352 635 248 635H247C181 635 134 604 106 573L56 506L82 482C117 529 151 557 201 557C246 557 287 531 287 466C287 404 228 364 143 336L150 302C165 306 186 309 204 309C258 309 324 275 324 179C324 85 285 44 235 44C175 44 128 88 106 108C92 118 80 116 69 106C56 97 39 79 39 61C39 47 46 34 63 21C83 5 123 -12 174 -12C236 -12 454 58 454 222C454 297 403 362 306 380V382Z" id="g13-49"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,30.185,0)"><path d="M152 515H407L439 613L433 623H115L70 303C91 310 134 322 179 322C270 322 327 264 327 174C327 100 297 45 235 45C178 45 130 84 106 106C93 117 82 120 70 112C53 99 38 76 38 61S43 36 63 20C84 2 126 -12 166 -12C226 -11 297 15 354 56C424 105 455 164 455 226C455 312 382 394 254 394C228 394 175 380 125 362L152 515Z" id="g13-51"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0135,0,0,-0.0135,37.098,0)"><path d="M253 635C100 635 24 509 24 420C24 314 97 231 217 231C234 231 251 236 275 248L324 274C298 154 195 47 47 21L53 -15C98 -14 184 2 223 18C365 74 471 201 471 382C471 519 398 635 253 635ZM232 595C310 595 335 485 335 387C335 365 334 340 330 313C316 307 300 300 275 300C202 300 156 374 156 460C156 525 178 595 232 595Z" id="g13-55"></path><glyph.data ascent="1024" descent="-360" horiz-adv-x="500" vert-adv-y="500"></glyph.data></g><g transform="matrix(.0095,0,0,-0.0095,43.872,-5.984)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z" id="g50-43"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="611" vert-adv-y="611"></glyph.data></g></svg></span></td><td align="center">1.576797</td></tr><tr><td align="left">(6) Yeast extract</td><td align="center">−0.49564</td><td align="center">−0.247821</td></tr><tr><td align="left">(7) Urea</td><td align="center">−1.90632</td><td align="center">−0.953159</td></tr><tr><td align="left">(8) Glucose</td><td align="center">−1.78105</td><td align="center">−0.890523</td></tr><tr><td align="left">(9) Starch</td><td align="center">0.50654</td><td align="center">0.253268</td></tr><tr><td align="left">(10) Manganese sulfate</td><td align="center">−0.43573</td><td align="center">−0.217865</td></tr><tr><td align="left">(11) Monobasic potassium phosphate</td><td align="center">0.96405</td><td align="center">0.482026</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td><svg height="10.5763pt" id="M22" style="vertical-align:-0.04990005pt" version="1.1" viewbox="-0.0498162 -10.5264 6.41401 10.5763" width="6.41401pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0095,0,0,-0.0095,0,-5.984)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z" id="g50-43"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="611" vert-adv-y="611"></glyph.data></g></svg>Significant effect.<br/></td></tr></table>

Results and coefficient effect for the production of tannase presented by the variables used in the Plackett-Burman design (PB).

The Scientific World Journal

tab4

Table 4

Table 4: Production, Characterization of Tannase from <i>Penicillium montanense</i> URM 6286 under SSF Using Agroindustrial Wastes, and Application in the Clarification of Grape Juice (<i>Vitis vinifera</i> L.) 

Table 4 | Production, Characterization of Tannase from Penicillium montanense URM 6286 under SSF Using Agroindustrial Wastes, and Application in the Clarification of Grape Juice (Vitis vinifera L.) 