Biobleaching of Industrial Important Dyes with Peroxidase Partially Purified from Garlic

Table 1

Purification table of garlic peroxidase.

Purification step	Volume of enzyme (mL)	Protein conc. (mg/mL)	Peroxidase activity (U/mL)	Specific activity (U/mg)	Total activity (U)	Purification fold	% yield
Crude enzyme	2000	4.981	20.39	4.09	40,780	1	100
(NH4)2SO4 precipitation	300	5.669	27.70	4.89	8,310	1.2	20.4
Dialyzed enzyme	180	2.650	41.78	15.77	7,520	3.9	90.5
Gel filtration	30	2.068	52.23	25.26	1,567	6.2	21