Characterisation of Drosophila Ubx^CPTI000601 and hth^CPTI000378 Protein Trap Lines

Figure 1

Ubx-YFP expression patterns during embryogenesis. ((a)–(d)) Ubx-YFP labelling; (a) Stage 10 embryo: Ubx-YFP expression is detected in PS5–13. The indicated numbers refer to parasegments; (b) Stage embryo: Ubx-YFP is expressed in the ectoderm in PS5–13. Ubx-YFP shows heterogeneous patterns of expression both within and between the parasegments; arrowheads mark parasegment boundaries; (c) Stage 15; (d) Stage 16. ((e)-(f)) Similar expression is observed for the endogenous Ubx gene detected by immunostaining in wild type embryos using α-Ubx antibody; (e) Stages ; (f) Stage 15. ((g)–(g′′)) Double-labelling of Ubx-YFP and its endogenous gene using α-GFP and α-Ubx antibodies, respectively, in Ubx ^CPTI000601 embryos. ((h)–(h′′)) a high magnification of a region boxed in (g). Double-labelling shows that the protein trap has very similar temporal and spatial expression patterns to its endogenous gene. All embryos are viewed laterally with anterior to the left.