Characterisation of Drosophila Ubx^CPTI000601 and hth^CPTI000378 Protein Trap Lines

Figure 4

Patterns of Hth-YFP expression. ((a)–(e)) Left panels: Hth-YFP embryos, stained with α-GFP antibodies; Hth-YFP expression is indicated by purple arrowheads. Right panels: the distribution of the endogenous Hth protein for the corresponding embryonic stages in the wild type. (a) Lateral view of a Stage 9 embryo, PR: procephalon, gb: germ band. (b) Dorsal view of a Stage 10 embryo. A: anterior and P: posterior. (c) Stage 11 embryo; Hth-YFP is strongly expressed anteriorly but declines posteriorly. (d) Stage 13 embryo. The posterior extent of the CNS is indicated by the yellow arrow. (e) Late stage of embryogenesis. Hth-YFP expression is prominent in the CNS of the Stage 15 and Stage 16 embryos but becomes weaker in the posterior regions. ((f)–(i′′)) Hth-YFP embryos stained with α-GFP and α-Hth antibodies. ((g)–(g′′)) High magnification of a region boxed in (f), whereas ((i)–(i′′)) are high magnifications of a region boxed in (h). Overall, the protein trap shows similar temporal and spatial expression patterns to the endogenous Hth protein in the embryo.