Research Article

Characterisation of Drosophila UbxCPTI000601 and hthCPTI000378 Protein Trap Lines

Figure 5

The patterns of Hth expression in T2 and T3 imaginal discs. ((a)–(c)), ((d)–(f)), and ((g)–(i)) are T2 wing, T3 leg, and T3 haltere discs, respectively. ((a), (d), and (g)) The expression patterns of endogenous Hth; ((b), (e), and (h)) the expression patterns of Hth-YFP; ((c), (f), and (i)) the expression patterns of Hth-YFP in the boxed regions in the corresponding images at high magnification showing that the protein trap is nuclear. Double-labelling of Hth-YFP and its endogenous gene shows that they have very similar temporal and spatial expression patterns in wing ((j)–(j′′)), haltere ((k)–(k′′)), and T3 leg ((l)–(l′′)) imaginal discs. ((m)–(m′′)) are higher magnifications of the boxed region in (l).