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The Scientific World Journal
Volume 2014, Article ID 191535, 14 pages
http://dx.doi.org/10.1155/2014/191535
Research Article

Characterisation of Drosophila UbxCPTI000601 and hthCPTI000378 Protein Trap Lines

1Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
2Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia
3Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK
4Cambridge Systems Biology Centre, University of Cambridge, Downing Street, Cambridge CB2 EH, UK

Received 19 May 2014; Accepted 1 August 2014; Published 15 October 2014

Academic Editor: Makoto Sato

Copyright © 2014 Siew Woh Choo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

UbxCPTI000601 and hthCPTI000378 are YFP protein trap insertions in Ubx and hth, respectively. In the case of Ubx, the YFP exon is inserted into the last intron of the gene at genomic position chr3R:12486327. The inserted exon is in the same frame as all six known alternatively spliced transcript variants of Ubx. The hthCPTI000378 line is an insertion at genomic position chr3R:6381126 in the endogenous hth gene. The insertion traps all but the two shortest hth spliced transcript variants (hth-RE and hth-RF).

  1. Supplementary Material