The Scientific World Journal / 2014 / Article / Fig 1

Research Article

RNAi-Mediated Knockdown of IKK1 in Transgenic Mice Using a Transgenic Construct Containing the Human H1 Promoter

Figure 1

IKK1 silencing in HEK293T transiently transfected cells. (a) Sequences included in pSuper plasmid vector for the production of shRNAs designed to knockdown IKK1 expression. Two IKK1 specific oligonucleotides (A and B) and one control scrambled sequence (C) were cloned in pSuper and tested in transfected cells. Nucleotide numbers refer to RefSeq: MN_007700.2. (b) Northern blot analysis of transiently transfected HEK293T cells hybridized with a probe specific for IKK1. Plates were cotransfected with a plasmid for the expression of IKK1 and the indicated pSuper-derivatives; “none” indicates that an empty plasmid was used. (c) Western blot analysis of IKK1 produced in transfected cells. In (b) and (c), each lane contains pooled RNAs or proteins extracts from two different plates. Ratios between the IKK1 expression plasmid and the pSuper-derived interfering plasmid used in each transfection are indicated below the blots in (b) and (c).

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