RNAi-Mediated Knockdown of IKK1 in Transgenic Mice Using a Transgenic Construct Containing the Human H1 Promoter
Figure 2
Generation of H1-IKK1-siRNA transgenic mice. (a) Schematic representation of the transgenes microinjected in one-cell mouse embryos. Transgene A1 is a 285 bp fragment containing the H1 promoter (white box) and the DNA oligonucleotide for shRNA synthesis (filled in black). Transgene A2 contained, in addition, the rest of the pSuper plasmid in its 3′ end (3234 bp total length). White arrows indicate the primers used for PCR-genotyping of transgenic mice. Relevant restriction sites are indicated. (b) Southern blot analysis of transgenic founders. The transgene injected (A1 or A2) and the name of the transgenic lines (L1 to L7) are indicated. Asterisks indicate founder mice that did not transmit the transgene to their progeny and were not included in further analysis. In the lane marked as C it was loaded DNA from a nontransgenic control mouse and the amount of fragment A2 corresponding to 20 copies per haploid genome. Figures in the left of the blot indicate fragment size in kbp.
