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The Scientific World Journal
Volume 2014 (2014), Article ID 231293, 10 pages
http://dx.doi.org/10.1155/2014/231293
Research Article

Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry

1Bioresourres Regional Innovation Center, Soonchunhyang University, Asan 336-745, Republic of Korea
2Industry-Academy Cooperation Foundation, Soonchunhyang University, Asan 336-745, Republic of Korea
3Korea Institute of Toxicology, Korea Research Institute of Chemical Technology, Daejeon 305-343, Republic of Korea
4College of Pharmacy, Dankook University, Cheonan 330-714, Republic of Korea
5Department of Pharmacology, College of Medicine, Dankook University, Cheonan 330-714, Republic of Korea
6Dankook Translational Research Center, Dankook University, Cheonan 330-714, Republic of Korea

Received 31 August 2013; Accepted 7 November 2013; Published 30 January 2014

Academic Editors: C. M. Davis and F. S. Hall

Copyright © 2014 Tae-Hyun Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Platycodon grandiflorum has long been used as a traditional oriental medicine for respiratory disorder. Platycodin D (PD) is known as the main component isolated from the root of PG. A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitation of PD in rat plasma. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization and multiple reaction monitoring in positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of PD were linear over the concentration range of 50–10,000 ng/mL in rat plasma. The coefficient of variation and relative error at five QC levels were 1.0 to 8.8% and 0.7 to 8.7%, respectively. After a single oral administration of 500 mg/kg and a single intravenous administration of 25 mg/kg of 3% PD extract (a PG extract including 3% of PD), platycodin D and platycodin D3 were detected and pharmacokinetic parameters were estimated. The oral bioavailability of platycodin D and platycodin D3 was 0.29% and 1.35% in rats at 500 mg/kg of 3% PD extract of PG, respectively. The present method can be applied to pharmacokinetic analysis of platycodins and platycosides of the PG.