The Reproducibility of Indoor Air Pollution (IAP) Measurement: A Test Case for the Measurement of Key Air Pollutants from the Pan Frying of Fish Samples

Table 2

Details of the sample, sampling, and analytical system for the analysis of smoke released from mackerel samples.

(a) Information of mackerel sample

Order	Sample code	Weight (g)	Room Temperature (°C)	Sampling
				Date	Time
1	M1	192	15.3	27 Jan. 2014	10:03
2	M2	169	17.0	27 Jan. 2014	13:10
3	M3	200	16.5	27 Jan. 2014	16:11
4	M4	194	16.1	27 Jan. 2014	19:03
5	M5	186	16.2	28 Jan. 2014	10:20
6	M6	167	16.9	28 Jan. 2014	13:31
7	M7	184	16.4	28 Jan. 2014	16:07
8	M8	189	15.8	28 Jan. 2014	19:09
9	M9	189	17.1	29 Jan. 2014	11:08
10	M10	191	16.5	29 Jan. 2014	14:10
11	M11	195	15.9	29 Jan. 2014	17:07

(b) Information of sampling

(1) Target compounds:	Gaseous VOCs (𝑛=12)
(2) Sampling approach:	Lung sampling
(3) Sample container:	10 L polyester aluminum (PEA) bag
(4) Sampling flow rate:	20 L min^−1
(5) Sampling time:	0.5 min
(6) Sample volume:	10 L

(c) Analytical method

Order	Target compounds	Pretreatment	Separation system	Detector
1	Particulate matters (𝑛=4)	—	—	90° light-scattering laser photometer
2	FA	DNPH-cartridge	HPLC: Acclaim 120 C18	UV
	AA
3	Aldehyde (4)	Sorbent tube/thermal desorption	GC: wax column (30 m × 0.25 mm × 0.25 μm)	Quadrupole mass spectrometry
	Aromatics (4)
	Ketone (1)
	Ester (1)

(d) Operational description of two instrumental systems (HPLC and GC) in this study

(A) HPLC/UV (Spectrasystem UV2000, Thermo scientific, USA) system for carbonyl compounds analysis
(i) Injector		(iii) Detector (UV)
Volume:	20 μL	Wavelength:	360 nm
(ii) Pump		(iv) Column (C18, Hichrom, UK)
Flow rate:	1.5 mL/min	Length:	250 mm
Mobile phase:	70 : 30 acetonitrile : H2O	Diameter:	4.6 mm
Analysis time:	16 min	Particle size:	5 μm
(B) TD-GC/MS system
(1) GC (SHIMADZU GC-2010, JAPAN) and MS (SHIMADZU GCMS-QP2010, JAPAN) system
(i) Oven		(ii) Detector (MS)
1st oven temperature:	40°C (5 min.)	Ionization mode:	EI (70 eV)
1st oven rate:	5°C min^−1	Ion source temperature:	230°C
2nd oven temperature:	220°C (5 min.)	Interface temperature:	230°C
Total time:	46 min	TIC scan range:	35~600 M z^−1
Carrier gas:	He (99.999%)
Column flow:	1 mL min^−1
(2) Thermal desorber (UNITY II, Markes International, Ltd., UK) condition
Cold trap:	Carbopack C + Carbopack B (volume ratio = 1 : 1)
Split ratio:	1 : 5	Trap temp (low):	5°C
Split flow:	5 mL min^−1	Trap temp (high):	330°C
Trap hold time:	5 min	Flow path temperature:	180°C