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The Scientific World Journal
Volume 2014 (2014), Article ID 239208, 7 pages
http://dx.doi.org/10.1155/2014/239208
Research Article

A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata Lectin

1Laboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil
2Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil
3Laboratory of Biologically Actives Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60440-970 Fortaleza, CE, Brazil
4Institute of Chemical and Geosciences, Federal University of Pelotas Institute of Chemical and Geosciences, Federal University of Pelotas, 96160-000 Pelotas, RS, Brazil
5Computer Engineering of Sobral, Federal University of Ceará, 62042-280 Sobral, CE, Brazil

Received 30 May 2014; Accepted 2 October 2014; Published 5 November 2014

Academic Editor: Alberto A. Iglesias

Copyright © 2014 Juliana Montezuma Barbosa Monteiro Tínel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Leishmaniasis is a vector-borne disease transmitted by phlebotomine sand fly. Susceptibility and refractoriness to Leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. Promastigote attachment to sand fly midgut epithelium is essential to avoid being excreted together with the digested blood meal. Promastigote and gut sand fly surface glycans are important ligands in this attachment. The purpose of the present study was to evaluate the interaction of three lectins isolated from leguminous seeds (Diocleinae subtribe), D-glucose and D-mannose-binding, with glycans on Lutzomyia migonei midgut. To study this interaction the lectins were labeled with FITC and a fluorescence assay was performed. The results showed that only Dioclea violacea lectin (DVL) was able to interact with midgut glycans, unlike Cratylia floribunda lectin (CFL) and Canavalia gladiata lectin (CGL). Furthermore, when DVL was blocked with D-mannose the interaction was inhibited. Differences of spatial arrangement of residues and volume of carbohydrate recognition domain (CRD) may be the cause of the fine specificity of DVL for glycans in the surface on Lu. migonei midgut. The findings in this study showed the presence of glycans in the midgut with glucose/mannose residues in its composition and these residues may be important in interaction between Lu. migonei midgut and Leishmania.