The Scientific World Journal / 2014 / Article / Fig 2

Research Article

The Role of Hibiscus sabdariffa L. (Roselle) in Maintenance of Ex Vivo Murine Bone Marrow-Derived Hematopoietic Stem Cells

Figure 2

Effect of Roselle supplementation at various concentrations on the survival of Sca-1+ cells that represents subpopulation of mouse hematopoietic stem cells. (a) Representative flow cytograms of cells harvested from control (A) and 24 hours of Roselle supplemented groups (B–G). Cells were treated with anti-mouse FITC-Sca-1 monoclonal antibody and expression of surface antigen Sca-1 was analysed using a flow cytometer. (b) The percentage of Sca-1 expression from various experimental groups and (c) absolute counts of Sca-1+ cells from various experimental groups. The values are the mean ± SEM of 3 replicates. indicates significant increase () as compared to the control group.