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The Scientific World Journal
Volume 2014, Article ID 263642, 7 pages
http://dx.doi.org/10.1155/2014/263642
Research Article

A Novel In Vitro Protocol for Inducing Direct Somatic Embryogenesis in Phalaenopsis aphrodite without Taking Explants

Department of Life Sciences, National University of Kaohsiung, Kaohsiung 811, Taiwan

Received 9 February 2014; Accepted 28 April 2014; Published 15 May 2014

Academic Editor: Jean-Francois Hausman

Copyright © 2014 Jia-Hua Feng and Jen-Tsung Chen. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. Arditti and R. Ernst, Eds., Micropropagation of Orchids, John Wiley & Son, New York, NY, USA, 1993.
  2. I. K. Vasil, “A history of plant biotechnology: from the cell theory of Schleiden and Schwann to biotech crops,” Plant Cell Reports, vol. 27, no. 9, pp. 1423–1440, 2008. View at Publisher · View at Google Scholar · View at Scopus
  3. J. L. Zimmerman, “Somatic embryogenesis: a model for early development in higher plants,” Plant Cell, vol. 5, no. 10, pp. 1411–1423, 1993. View at Publisher · View at Google Scholar · View at Scopus
  4. J. E. Thompson, R. L. Legge, and R. F. Barber, “The role of free radicals in senescence and wounding,” New Phytologist, vol. 105, pp. 317–344, 1987. View at Google Scholar
  5. W. H. Ko, C. C. Su, C. L. Chen, and C. P. Chao, “Control of lethal browning of tissue culture plantlets of Cavendish banana cv. formosana with ascorbic acid,” Plant Cell, Tissue and Organ Culture, vol. 96, no. 2, pp. 137–141, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. C. Chang and W. C. Chang, “Plant regeneration from callus culture of Cymbidium ensifolium var. misericors,” Plant Cell Reports, vol. 17, no. 4, pp. 251–255, 1998. View at Publisher · View at Google Scholar · View at Scopus
  7. Y.-C. Chen, C. Chang, and W.-C. Chang, “A reliable protocol for plant regeneration from callus culture of Phalaenopsis,” In Vitro Cellular and Developmental Biology—Plant, vol. 36, no. 5, pp. 420–423, 2000. View at Google Scholar · View at Scopus
  8. Y.-H. Lin, C. Chang, and W.-C. Chang, “Plant regeneration from callus culture of a Paphiopedilum hybrid,” Plant Cell, Tissue and Organ Culture, vol. 62, no. 1, pp. 21–25, 2000. View at Publisher · View at Google Scholar · View at Scopus
  9. T.-Y. Chen, J.-T. Chen, and W.-C. Chang, “Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids,” Plant Cell, Tissue and Organ Culture, vol. 76, no. 1, pp. 11–15, 2004. View at Publisher · View at Google Scholar · View at Scopus
  10. H.-H. Chung, J.-T. Chen, and W.-C. Chang, “Cytokinins induce direct somatic embryogenesis of Dendrobium Chiengmai Pink and subsequent plant regeneration,” In Vitro Cellular and Developmental Biology—Plant, vol. 41, no. 6, pp. 765–769, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. J. T. Chen and W. C. Chang, “Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis,” Biologia Plantarum, vol. 50, no. 2, pp. 169–173, 2006. View at Publisher · View at Google Scholar · View at Scopus
  12. P.-I. Hong, J.-T. Chen, and W.-C. Chang, “Plant regeneration via protocorm-like body formation and shoot multiplication from seed-derived callus of a maudiae type slipper orchid,” Acta Physiologiae Plantarum, vol. 30, no. 5, pp. 755–759, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. W.-P. Gow, J.-T. Chen, and W.-C. Chang, “Effects of genotype, light regime, explant position and orientation on direct somatic embryogenesis from leaf explants of Phalaenopsis orchids,” Acta Physiologiae Plantarum, vol. 31, no. 2, pp. 363–369, 2009. View at Publisher · View at Google Scholar · View at Scopus
  14. H.-L. Kuo, J.-T. Chen, and W.-C. Chang, “Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis 'Little Steve',” In Vitro Cellular and Developmental Biology—Plant, vol. 41, no. 4, pp. 453–456, 2005. View at Publisher · View at Google Scholar · View at Scopus
  15. R. L. Taylor, “The foliar embryos of Malaxis paludosa,” Canadian Journal of Botany, vol. 45, pp. 1553–1556, 1967. View at Google Scholar
  16. J. A. Yarbrough, “Anatomical and developmetal studies of the foliar embryos of Bryophyllum calcynum,” The American Journal of Botany, vol. 19, pp. 443–453, 1932. View at Google Scholar
  17. J.-T. Chen and W.-C. Chang, “Induction of repetitive embryogenesis from seed-derived protocorms of Phalaenopsis amabilis var. formosa Shimadzu,” In Vitro Cellular and Developmental Biology—Plant, vol. 40, no. 3, pp. 290–293, 2004. View at Publisher · View at Google Scholar · View at Scopus
  18. M.-J. Lin and B.-D. Hsu, “Photosynthetic plasticity of Phalaenopsis in response to different light environments,” Journal of Plant Physiology, vol. 161, no. 11, pp. 1259–1268, 2004. View at Publisher · View at Google Scholar · View at Scopus
  19. C.-C. Chang, H.-C. Lin, I.-P. Lin et al., “The chloroplast genome of Phalaenopsis aphrodite (Orchidaceae): comparative analysis of evolutionary rate with that of grasses and its phylogenetic implications,” Molecular Biology and Evolution, vol. 23, no. 2, pp. 279–291, 2006. View at Publisher · View at Google Scholar · View at Scopus
  20. W.-H. Chen, Y.-C. Tseng, Y.-C. Liu et al., “Cool-night temperature induces spike emergence and affects photosynthetic efficiency and metabolizable carbohydrate and organic acid pools in Phalaenopsis aphrodite,” Plant Cell Reports, vol. 27, no. 10, pp. 1667–1675, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. W.-P. Gow, J.-T. Chen, and W.-C. Chang, “Influence of growth regulators on direct embryo formation from leaf explants of Phalaenopsis orchids,” Acta Physiologiae Plantarum, vol. 30, no. 4, pp. 507–512, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. W.-P. Gow, J.-T. Chen, and W.-C. Chang, “Enhancement of direct somatic embryogenesis and plantlet growth from leaf explants of Phalaenopsis by adjusting culture period and explant length,” Acta Physiologiae Plantarum, vol. 32, no. 4, pp. 621–627, 2010. View at Publisher · View at Google Scholar · View at Scopus
  23. W.-H. Chen, C.-Y. Tang, T.-Y. Lin, Y.-C. Weng, and Y.-L. Kao, “Changes in the endopolyploidy pattern of different tissues in diploid and tetraploid Phalaenopsis aphrodite subsp. formosana (Orchidaceae),” Plant Science, vol. 181, no. 1, pp. 31–38, 2011. View at Publisher · View at Google Scholar · View at Scopus
  24. C.-L. Su, Y.-T. Chao, Y.-C. Alex Chang et al., “De novo assembly of expressed transcripts and global analysis of the Phalaenopsis aphrodite transcriptome,” Plant and Cell Physiology, vol. 52, no. 9, pp. 1501–1514, 2011. View at Publisher · View at Google Scholar · View at Scopus
  25. W. J. Guo, Y. Z. Lin, and N. Lee, “Photosynthetic light requirements and effects of low irradiance and daylength on Phalaenopsis amabilis,” Journal of American Society For Horticultural Science, vol. 137, pp. 465–472, 2012. View at Google Scholar
  26. Y.-Y. Hsiao, Z.-J. Pan, C.-C. Hsu et al., “Research on orchid biology and biotechnology,” Plant and Cell Physiology, vol. 52, no. 9, pp. 1467–1486, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. Y. C. Liu, K. M. Tseng, C. C. Chen et al., “Warm-night temperature delays spike emergence and alters dawn-dusk changes in carbon pool metabolism in the stem and leaves of Phalaenopsis aphroide,” Scientia Horticulturae, vol. 161, pp. 198–203, 2013. View at Google Scholar
  28. C. L. Su, W. C. Chen, A. Y. Lee et al., “A modified ABCDE model of flowering in orchids based on gene expression profiling studies of the moth orchid Phalaenopsis aphrodite,” PLoS ONE, vol. 8, article e80462, 2013. View at Google Scholar
  29. T. Murashige and F. Skoog, “A revised medium for rapid growth and bioassays with tobacco tissue cultures,” Physiologia Plantarum, vol. 15, pp. 495–497, 1962. View at Google Scholar
  30. D. B. Duncan, “Multiple range and multiple F test,” Biometrics, vol. 11, pp. 1–42, 1955. View at Google Scholar
  31. J. T. Chen, C. Chang, and W. C. Chang, “Direct somatic embryogenesis on leaf explants of Oncidium “Gower Ramsey” and subsequent plant regeneration,” Plant Cell Reports, vol. 19, no. 2, pp. 143–149, 1999. View at Google Scholar · View at Scopus
  32. G. E. Sim, C. S. Loh, and C. J. Goh, “High frequency early in vitro flowering of Dendrobium Madame Thong-In (Orchidaceae),” Plant Cell Reports, vol. 26, no. 4, pp. 383–393, 2007. View at Publisher · View at Google Scholar · View at Scopus
  33. T. L. Arnaldos, R. Muñoz, M. A. Ferrer, and A. A. Calderón, “Changes in phenol content during strawberry (Fragaria × ananassa, cv. Chandler) callus culture,” Physiologia Plantarum, vol. 113, no. 3, pp. 315–322, 2001. View at Publisher · View at Google Scholar · View at Scopus
  34. H. Laukkanen, H. Häggman, S. Kontunen-Soppela, and A. Hohtola, “Tissue browning of in vitro cultures of Scots pine: role of peroxidase and polyphenol oxidase,” Physiologia Plantarum, vol. 106, no. 3, pp. 337–343, 1999. View at Publisher · View at Google Scholar · View at Scopus
  35. M. Zapprometov, “The formation of phenolic compounds in plant cell and tissue cultures and possibility of its regulation,” Advances in Cell Cultutre, vol. 7, pp. 240–245, 1989. View at Google Scholar
  36. V. I. Kefeli, M. V. Kalevitch, and B. Borsari, “Phenolic cycle in plants and environment,” Journal of Cell and Molecular Biology, vol. 2, pp. 13–18, 2003. View at Google Scholar