A Novel In Vitro Protocol for Inducing Direct Somatic Embryogenesis in Phalaenopsis aphrodite without Taking Explants

Table 2

Number of embryos formed on the distal site of shoot of 4-month-old seedlings, depending on culture systems and concentration of cytokinins.

PGR treatments (mg L^−1)	Solid medium	Two-layer medium	Two-step culture
PGR-free	0^c*	5.6^b	0^c
BA
1	2.4^abc	11.8^a	5.2^a
2.5	2.2^abc	10.0^ab	1.6^bc
5	1.6^bc	8.8^ab	1.6^bc
TDZ
0.1	3.2^abc	11.8^a	2.4^bc
0.5	6.6^a	10.2^ab	3.0^ab
1.0	6.2^ab	10.8^ab	2.2^bc

∗Data in the same column followed by the same letters are not significantly different by Duncan’ multiple range test at P < 0.05.