Research Article

A Novel In Vitro Protocol for Inducing Direct Somatic Embryogenesis in Phalaenopsis aphrodite without Taking Explants

Table 3

Number of embryos formed on the leaf surface of 2-month-old seedlings, depending on culture systems and concentration of cytokinins.

PGR treatments
(mg L−1)
Solid mediumTwo-layer medium Two-step culture

PGR-free0a*2.6a19.0a
BA
 10a3.6a20.6a
 2.50a4.6a10.2a
 50a5.0a19.4a
TDZ
 0.10a6.0a16.2a
 0.50a7.0a22.6a
 1.00a5.6a15.2a

Data in the same column followed by the same letters are not significantly different by Duncan’ multiple range test at P < 0.05.